DeepChek® Assay Whole Genome HIV-1 Genotyping

The DeepChek® Assay Whole Genome HIV-1 Genotyping is intended to be used for amplifying whole-genome of the human immunodeficiency virus type one (HIV-1) from input RNA extracted from plasma, serum or whole blood samples. In clinical samples where HIV-1 is detected, the DeepChek® Assay Whole Genome HIV-1 Genotyping might aid in the determination of antiviral resistance with the detection of resistance associated mutations in HIV-1 targeted genes (e.g., reverse transcriptase, protease, integrase, envelope, …).

Features

  • 2 rt-PCR steps test using extracted RNA from HIV-1 positive patients followed by NGS.

  • Used for amplifying whole-genome of the human immunodeficiency virus type one (HIV-1) for the determination of antiviral resistance associated mutations in HIV-1 targeted genes including new entry & capsid inhibitors.

  • Inclusivity: excellent coverage of all representative HIV-1 subtypes from M group strains (A1, A2, B, C, D, F1, F2, G, H, J, K and L).

  • LoD: 25'000 cp/mL

  • Validated with Roche MagNA Pure 24 instrument and reagents, Thermo Fisher Scientific ProFlex PCR System; (model 3 x 32) and Illumina iSeq-100 instrument with a run of 24 samples.

  • Shall be compatible with other magnetic-beads RNA isolation instruments and reagents, any PCR thermal cycler with ramp rate of ≥ 1°C/s and NGS instruments.

  • Shall be compatible with run of 48 / 96 and 384 samples to reach assay cut-off (minimal median total coverage of 1000 reads for the amplicons and a Phred Quality Score Q30>80% (or equivalent))

  • Push-button data analysis validated with the use of the ABL DeepChek® software (Whole genome HIV-1 (REF S-22-05 (WHM)). Shall be compatible with other downstream NGS analysis software tailored to HIV-1.

  • Same thermal cycling program in five (5) distinct wells

  • Coverage : ~ 9600 bp

  • Number of amplicons: 5x ~700 to 3500 bp

Examples of Reports

Ordering Informations

Product Reference
DeepChek® Assay Whole Genome HIV-1 Genotyping (CE-IVD)
170A24
DeepChek® Assay Whole Genome HIV-1 Genotyping (RUO)
170A24
DeepChek® HIV Software (CE-IVD)
S-12-023 (HL)

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