The DeepChek® Assay -HIV Integrase Genotyping and Drug Resistance 
is intended to be used for HIV-1 genotyping and provides antiretroviral susceptibility information for protease inhibitors (PI) and reverse transcriptase inhibitors (NRTI, NNRTI). It combines target-specific PCR reagents with in vitro diagnostic software both compatible with either Sanger or Next Generation Sequencing platforms..
Features & Benefits
| Technology | Security | Main Features | Additional Features | Services |
|---|---|---|---|---|
|
• Web-based (browser
only)
• Software & database
• Local or Cloud (+HDS)
hosting
• Unlimited user
accounts
•Unlimited analyses
|
• Data access restriction
(pools, read-only
mode…)
•Logging of user
accesses
•Encrypted database
• Reports validation
|
• SANGER data
management (AB1,
FASTA) with
embedded
chromatogram editor
• NGS data
management (FASTQ)
with dedicated
pipeline
• Genotyping (per
sample & cumulative)
• Subtyping
• Virtual-phenotyping
• Drug resistance
through up-to-date
guidelines
• GSS determination
and regiment ranking
• Tropism
• Reporting & labelling
|
• Report customization
• Contamination check
• Quality control
• Export (reports,
FASTA, XML…)
• Batch mode analysis
• Data mining
|
• Constant updates
• Annual upgrades
(versions)
• Historical data import
• LIMS integration
• HIS integration
• Support
• Trainings |
Characteristics & Performances
|
Compatibility with Capillary Electrophoresis (SANGER) platforms |
Any |
|
Compatibility with Next Generation Sequencing platforms |
Any |
|
Intended use |
CE-IVD – ISO-9001 manufacturing |
|
Types of samples |
Serum, plasma, pro-viral DNA, DBS |
|
Format |
24 samples/kit |
|
Content |
RT-PCR & Nested-PCR reagents (enzymes, master mixes, primers, dNTPs...), SANGER sequencing primers, protocols for SANGER and NGS sequencing, SANGER or NGS software analyses for 24 samples through a Cloud access |
|
Sensitivity |
1000 cp/mL for 400 µL plasma/serum. Protocols for low viral loads (>200 - 300 cp/mL) available |
|
Specificity |
Validated on most of the subtypes and CRF strains |
|
Reproducibility |
>99% |
|
Workflow |
From sample to result in ~15 hours for SANGER and ~30 hours for NGS (depending on the platform) |
|
Covered positions |
PR: codons 1 to 99, RT: codons 1 to 320 |
|
Compatible extraction methods |
Automatic (MagNA Pure Compact Nucleic Acid Isolation Kit I - Roche, Promega - Abbott), Manual (Manual extractions using QIAamp® Viral RNA - Qiagen)... |
|
Data analysis and interpretation software |
Included (DeepChek-HIV / CE-IVD) |
|
Available analyses |
Subtyping, amino-acid mutations detection, nucleotide changes detection, drug resistance, NGS run quality report, GSS, clinical genotyping report... |
|
Drug resistance |
Flexible, through up to 7 different up-to-date guidelines including HIVdb/Stanford, ANRS, geno2pheno, Rega,... |
|
Included services |
Unlimited updates & upgrades of the software, support, training... |
|
Options |
Local servers, historical data import, integration with LIS and HIS, integration with sequencers, automation of the IT workflow, customization... |
Exemples of Reports
For NGS Sequencing (DeepChek®-HIV CE-IVD)
For SANGER Sequencing (Viroscore® CE-IVD)
Ordering Information
| Product | Reference |
|---|---|
|
DeepChek® Assay PROTEASE / REVERSE TRANSCRIPTASE Genotyping and Drug Resistance (CE-IVD)
|
121A24 |
|
DeepChek® HIV Software (CE-IVD) |
S-12-023 (HL) |
|
ViroScore-HIV Annual Software License (CE-IVD) |
S-09-014 |
Downloads
Leaflet