ULTRAGENE™-HPV One qPCR Kit

The UltraGene ™HPV One qPCR Kit uses probe based qPCR technology to detect HPV with high specificity. Each sequence specific probe contains a 5 fluorophore and a 3 quencher. On its own, the probe’s 3 quencher effectively absorbs the emission from the nearby 5 fluorophore so that no net fluorescence is detected During PCR amplification, the probe binds to its specific target template While replicating the DNA sample, the PCR Taq Polymerase’s 5 to 3 exonuclease activity also hydrolyzes the probe, releasing a free floating 5 fluorophore that is detectable by the qPCR machine and can be translated into an amplification plot This HPV One qPCR kit identifies 14 types of high risk HPV and a human ACTIN control using 4 fluorescent channels in one tube FAM, HEX, ROX and Cy 5 As a result, this kit can not only detect the 14 high risk HPV types, it is capable of distinguishing HPV 16 and 18 from the other types The internal control, human ACTIN, indicates the quality of extracted DNA.